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The Plasma Cleaner and its use in Electron Microscopy

Science is a field of knowledge that has been expanding throughout the years. From the beginning of the Greek civilization, it has been one of the areas of learning which humans always take part in, leading to a great progress in the field throughout time. Mankind’s dedication to science has also led to it expanding into a mastery that consists of many other branches of learning, such as zoology, botany, physics and so many more.

While it has grown into a vast field of study, science kept on growing. Scientists in various fields have been so eager to know more about the world, thus widening their knowledge by doing more studies that are more advanced. And for these advanced studies that humans have come up with equipment that can be used to gather information better. Thus humans invented the laboratory tools such as the electron microscope.

The electron microscope is a very useful tool in conducting research. It is capable of seeing objects that are a thousand times smaller. Apparently it has way higher magnification compared to the optical microscope. However, this tool is complicated and requires personnel training for you to be able to properly use it. As electron microscope has a very high magnification, it is important that the specimen that is to be scanned to be uncontaminated in order to conduct inspection more properly. Thus it is important that you use a de-contaminator after you prepare the specimen. And for this you would need a Plasma Cleaner.

Plasma technology can be applied to any electron microscopes and sometimes referred to as “in-situ plasma cleaners”. In electron microscopes, hydrocarbons contamination can be a problem as this affects the image resolution of the electron microscope. This typically appears as a dark scan mark. How scan marks appear? This happens when electron beam scan has induced carbon deposition and carbon been a very low secondary electron yield.

In the past, the only solution to remove hydrocarbon build up in electron microscope is using chamber/column baking, this is tedious and lengthy maintenance process done by service engineers. With plasma technology, an effective de-contaminator, hydrocarbon can be effectively removed from the sample surface within 6 minutes. As a best practice, users can now clean the chamber as often as they like to prevent hydrocarbon build up.

Therefore plasma cleaner has become an essential de-contaminator accessory in high resolution imaging applications.

Plasma Cleaner is a very important tool in electron microscopy. It is what you need in order to remove contamination from the specimen. With the aid of a plasma cleaner, your electron microscope is free from contamination. You can freely conduct your specimen inspection with the electron microscope without worries.

The value of equipment and electron microscopy in conducting research

Humanity has developed greatly all thanks to the discoveries that has been made in the past years.

Innovations in various fields of science paved so many pathways which led to our progress. Whether it is in the field of chemistry, biology, or physics, all the scientific knowledge which have been gathered by people who have spent years exerting not just effort, but also time and money, are now being used in different kinds of industries from car manufacturing to pharmaceutics.

Of course, all these scientific information that we have nowadays are all thanks to the valuable information that people gather when they conduct researches. And these research studies cannot be conducted without the aid of advanced equipment. One of these equipment would be the electron microscope, an advanced version of the simple microscope. This scientific instrument examines objects using electrons. An electron microscope has a higher magnification, typically capable of seeing things that are a thousand times smaller compared to an optical microscope. But unlike an ordinary microscope, electron microscopy comes with a large piece of equipment. Moreover, a trained personnel is needed in order to operate the machine. Among the many different kinds of electron microscope is the Scanning Electron Microscope, also known as SEM.

However, not all specimens are suitable to be put under the lens of electron microscope. Some specimens are non-conducting or poorly conducting, thus they make unsuitable samples to be inspected using the electron microscope. Fortunately there is a proper solution to this. A sample only has to undergo sputter coating in order for it to be viewable under an electron microscope.

Sputter coaters are relevant in conducting research using electron microscope. Sputter coaters serve as sample coating for specimen which were non-conductive or poorly conductive. It is the best method when you are inspecting specimen under a scanning electron microscope. Equipment is important when it comes to conducting studies.

Significance of Applied Physics in electron microscopy community

Electron microscope plays a crucial role in determining an image of the specimen. With electron microscopy that proffers higher magnifications enable to see such a smaller objects in detailed. Electron microscopy comes with a large piece of equipment as well as required a personnel training to operate the machine. Most of electron microscopes use electrostatic lenses to manage the route of electrons. At Applied Physics, we provide ranging products that cater your needs in the electron microscope community. Therefore, we strive for its excellence in providing the highest standard of products and services to our customer.

Coating of sample is necessary in the field of electron microscopy to enhance and improvise the imaging of samples. The 108C Auto Carbon Coater is designed specifically for coating non-conductive specimens and seen as the most effective sample coaters. The 108C is a compact system, easy to operate.

PIE stands for Plasma, Ion and Electron. It is founded in silicon valley and has more than 15 years of experience in developing advanced semiconductor capital equipment. PIE develops and owns its plasma technology and therefore applies the latest development in plasma for a range of products such as EM-Kleen, SEMI-Kleen remote plasma cleaner for in-situ plasma cleaning in electron microscope.

With the latest Tergeo Tabletop plasma cleaner, this provides plasma cleaning for all types of electron microscope samples. The Tergeo plasma cleaner is also capable of attaching TEM holder for sample cleaning.

The Cressington coaters are the most advanced coaters that available today with a huge range of applications such as TEM, FESEM, EDS and SEM.

Plasma cleaning of specimens before proceeding to electron microscopy has proven to be an effective method in alleviating specimen contamination. It has been widely report that “an oxygen plasma” is plausible in getting rid of hydrocarbon contamination.

Plasma cleaner is designed specifically to be a quick fix for contamination in any vacuum systems. SEMI KLEEN plasma cleaner has 100 watt RF. It is adjustable and with low EMI emission.

Advantages of sputter coating for the specimen

Sputter Coater is the best method for organizing non- conducting specimens for inspection in a scanning electron microscope (SEM). The manual sputter coater is an ideal and systematic system for sample coating. It comes with Au of non- conducting samples for “standard SEM imaging”. The economical desktop have provided the benefits that you can reap, which offers rapid pump down times, fine grain coatings as well as fully variable current control. The safety is guaranteed, and the thickness monitor is highly suggested for precise measurements.

At applied physics, we offer a low cost system that would facilitate your work process. Carbon coater is designed for managing specimens for electron microscopy. The primary applications for carbon coating in Electron microscopy are X-ray microanalysis.

Cressington coater has been widely used where coating thickness has been consistency with high resolution coater. The Cressington 208HR caters a quick fix to the circumstances when coating difficult samples.

The main features for this product are:

Precision Thickness Control, Multiple sample stage movements, variable chamber geometry.

Electron microscopy has been widely used for examining objects that can’t be seen with human eye. Electron microscope focuses beams of electron that offers a higher magnification.

The electron microscopy must be well prepared before placing in the microscope vacuum. There are varieties of specimen and analysis that included fixation, dehydration, and cryofixation.

To achieve the optimum results during microscopy electron, it is required to have a clean and well- prepared specimens. The besmirch specimen can cause a detrimental effect on the electron microscope analysis.

Therefore, the plasma cleaner is designed to clean the hydrocarbon contamination. To get rid of hydrocarbon contamination a mixture of 25% of oxygen and 75% of argon is found to be the effective ones. It was reported that 25% of oxygen is effective in slashing down of hydrocarbon.

Benefits of Plasma cleaner for the electron microscopy

Plasma Cleaner is imperative tool for the electron microscopy, as it prevents contamination from occurring and removes any prior contamination from the specimen. There are several main causes of triggering this hydrocarbon contamination during specimen preparation. The standard method was not proven to be effective in impeding the contamination. Therefore, to achieve positive results during electron microscopy of semiconducting materials, it is significant to have a proper and clean specimen. This specimen borne besmirch can exacerbate the ability to conduct accurate electron microscope analysis.

It has been widely reported that oxygen plasma is highly recommended in eliminating hydrocarbon contamination. Foremost to remove the hydrocarbon contamination, a mixture of 25% of oxygen and 75% of argon is touted to be effective solution. Meanwhile, Electron microscope is a type of microscope that focuses a beam of electron. Electron microscope is proven to be highly effective due to greater magnifications that have a higher resolving power than any other microscope. The electron microscope plays an important role of many laboratories. The researcher has utilized the electron microscope to examine biological materials, a variety of molecules as well as other various surfaces.

The Cressington 108 sputter coaters are designed “for routine sample preparation”. The design is compact, economical and easy to operate. The Cressington 108 sputter coater provides the choice of manual and automatic operation.

Carbon coater is one of the most advanced and prevalent sample coaters. The 108C Auto carbon coater uses “a novel evaporation supply.” The supply renders stellar stability and replicability. Low consumption of power enables a better outcomes and the evaporation source can be functioned in “pulse” or “constant” modes.

Applied Physics that cater your needs

Applied Physics Pte Ltd is committed in providing the highest quality that pertaining to the electron microscopy community. The team has a vast of both experience and knowledge in electron microscopy as well as microanalysis. We have always strived for its excellence in understanding customer’s plight and providing the best solutions for them. The electron microscope is an effective method for exploring the micro world, and particularly uses a beam of electron to gain detailed information and allowing to see a smaller object efficiently.
The electron microscope has enormous resolving power that offers effective solutions than the light microscopy. The solely benefits the electron microscope has to offer is immense depth of field that would ease your hurdles.
The Cressington 208 HR coater has now provided the solutions for covering difficult circumstances or sample for FE-SEM. The main feature of this product is:

● Wide choice of coating materials that allows a wide choice of target materials

● Wide range of operating pressures: The flexible adjustment of power allows operation at argon gas pressure at 0.02 to 0.005 mbar

● Compact modern bench top design: The design will facilitate the work process and simultaneously terminate need for floor space, water, specialized electrical connections

The sputter coater designated to scanning electron microscopy to prevent charging of the specimen. At applied physics, we offer sputter coater which is an economical, compact and easy to operate, that would facilitate the work process. The customers will reap benefits of our sputter coater such as increase thermal conduction, reduced sample charging, as well as preserve beam sensitive specimens.
Meanwhile, carbon coater is designed to prepare specimens for electron microscopy – by evaporating high purify carbon fiber, and there is no need for expensive vacuum evaporators.
SEMI-KLEEN plasma cleaner offers a cleaning solution for contamination control, and has been proven for reducing the risk of ion sputtering damage.